Product Description
Pipes
Application Notes
PIPES is use to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is also use to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer | Fluorescence microscopy | Electron microscopy
Specifications
SKU | 02194838-CF |
Alternate Names | Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperzine-N,N' bis(ethanesulfonic acid); 1,4-Piperazinediethanesulfonic acid |
Application Notes | PIPES is use to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is also use to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results. |
Base Catalog Number | 194838 |
Beilstein Registry Number | 817713 |
CAS # | 5625-37-6 |
EC Number | 227-057-6 |
Format | Powder |
Grade | Molecular Biology Grade |
Hazard Statements | H317 |
Molecular Formula | C8H18N2O6S2 |
Molecular Weight | 302.36 g/mol |
Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
pKa | pKa2: 6.8 at 25 °C; 6.66 at 37 °C(Lit.) |
Preparation Method | Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH. |
Purity | ≥99% |
Sterilization of Solutions | For sterilization it is recommended to filter sterilize using a 0.2 µm filter. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
UV Visible Absorbance | UV/VIS: OD260 (0.1M in 0.2M NaOH) ≤0.05; UV/VIS: OD280 (0.1M in 0.2M NaOH) ≤0.05 |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
Safety Data Sheets
English (United States)