Product Description
The RapidPURE™ Plasmid Mini Kit is designed to isolate up to 20 μg of plasmid DNA from 1.5 mL of an overnight bacterial culture. All steps can be carried out in either a microcentrifuge or vacuum manifold.
Application Notes
The RapidPURE™ Plasmid Mini Kit is designed to isolate up to 20 μg of plasmid DNA from 1.5 mL of an overnight bacterial culture for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Isolation of plasmid DNA
Specifications
| SKU | 112066200 |
| Alternate Names | Plasmid Miniprep Purification Kit |
| Application Notes | The RapidPURE™ Plasmid Mini Kit is designed to isolate up to 20 μg of plasmid DNA from 1.5 mL of an overnight bacterial culture for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. |
| Base Catalog Number | 2066200 |
| Components | Pre-Lysis Buffer|Alkaline Lysis Solution |Neutralizing Solution |RapidPURE™ Mini Salt Solution |Wash Solution |RapidPURE™ Turbo Cartridges |RapidPURE™ Turbo Recovery Tubes |CIRCLEGROW™ Broth Pouch(es). |
| Format | GLASSMILK™ imbedded RapidPURE™ Turbo SPIN column |
| Media Type | Enhanced LB Media |
| Pack Size | 60 Preps |
| Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Preparation Method | Before first use, add the 12 mL of 200 proof (100% ethanol) to the Wash Solution (12 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C. |
| Recommended Alternate | MiniPrep Express™ Matrix |
| Safety Symbol | GHS07 |GHS05 |
| Sample Type | Plasmid Prep |
| Sample Volume | 1.5 ml of overnight culture |
| Unit Definition | Yields up to 20 μg of High Copy Number Vector DNA Per 1.5 ml overnight culture typical |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
![MP COA [2066200] lot ID: 121419](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [2066200] lot ID: 121419")
Safety Data Sheets
English (United States)