Saponin
Application Notes
1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Hemolyze erythrocytes | Permeabilize cultured human intestinal epithelial cells and paraformaldehyde-saponin- fixed human fibroblasts | Surface-active agent
| SKU | 02102855-CF |
| Alternate Names | Sapogenin Glycosides |
| Application Notes | 1% of saponin solution can hemolyze erythrocytes. Saponin solutions have been used in the following ways: concentrations of 0.005%-0.01% were used to permeabilize cultured human intestinal epithelial cells after incubation for 30' prior to immunofluorescence; a 0.05% solution of saponin was reportedly used to permeabilize paraformaldehyde-saponin- fixed human fibroblasts for the staining of intracellular fibronectin by different types of peroxidase conjugates for light and electron microscopy; saponins, a surface-active agent, have also been used to lyse the outer membranes of Rous sarcoma viruses, cell membranes of chicken liver and erythrocytes of human and guinea pig. It also has been used as an adjuvant for vaccines. |
| Base Catalog Number | 102855 |
| Biochemical Physiological Actions | Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes |
| CAS # | 8047-15-2 |
| EC Number | 232-462-6 |
| Format | Powder |
| Grade | Practical Grade |
| Hazard Statements | H319-H335 |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
| RTECS Number | VQ1400000 |
| Safety Symbol | GHS07 |
| Source | Plant |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [102855] lot ID: 2735J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [102855] lot ID: 2735J")
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