粪便样本的成分在很大程度上取决于人类或动物的饮食,其中包括纤维、未消化的颗粒、胆红素、复合多糖和脂质等。 这些化合物会影响样品的裂解,从而影响核酸提取的得率和纯度。SPINeasy™ RNA Kit for Feces采用了先进的研珠法技术,可以提取各种类型粪便样本;特异性结合RNA的硅胶柱模搭配独特的抑制剂去除技术FastDNase I,可在1-2分钟内彻底去除DNA污染,进一步提高实验效率。
SPINeasy™ RNA Kit for Feces可在20分钟内提取不含DNA污染的高质量RNA,无需额外的抑制剂/DNA去除步骤,即可直接应用于qPCR和RT-qPCR等下游实验。
Performance
图1 SPINeasy™ RNA Kit for Feces适用于处理不同类型的粪便样本。使用SPINeasy™ RNA Kit for Feces 从不同类型的粪便样本中提取的RNA得率更高、260/280 和 260/230 比值均达到最佳水平,RNA片段的完整性也与竞品 Q 试剂盒相似或更好,这表明SPINeasy™ RNA Kit for Feces处理不同类型的粪便样本都具有很好的效果。
同竞品试剂盒Q相比,采用SPINeasy™ RNA Kit for Feces提取的粪便样本RNA中无DNA污染。
Unlike competitor Q kit, no cross-DNA contamination was observed in RNA samples extracted with the SPINeasy® RNA Kit for Feces. As shown in Agilent virtual gel (Figure 1, red rectangle) or agarose gel profile (Figure 2), some RNA samples extracted with the competitor Q kit contained DNA contamination; this highlights the outstanding performance of the SPINeasy® RNA Kit for Feces as compared to other similar commercial kit on the market.
Application Notes
SPINeasy™ RNA Kit for Feces可在20分钟内提取不含DNA污染的高质量RNA,无需额外的抑制剂/DNA去除步骤,即可直接应用于qPCR和RT-qPCR等下游实验。
Usage Statement
MP Biomedicals的产品仅供研究或进一步生产使用,不得直接用于人体,除非有特殊声明。如需了解更多信息,请联系我们的客户服务部门。
Key Applications
基因组,分子生物学,PCR,qPCR
SKU | 116556050 |
Application Notes | SPINeasy™ RNA Kit for Feces可在20分钟内提取不含DNA污染的高质量RNA,无需额外的抑制剂/DNA去除步骤,即可直接应用于qPCR和RT-qPCR等下游实验。 |
Base Catalog Number | 6556050 |
Brand | SPINeasy |
Components | Equilibration buffer (12 mL), Lysing Matrix YB (50 ea), Phenol: chloroform: isoamyl alcohol (6 mL), Buffer FR1 (40 mL), Buffer FR2 (15 mL), Buffer FR3 (48 mL), Buffer FR4 (72 mL), Buffer FR5 (36 mL), RNase-free water (10 mL), FastDNase I enzyme (0.24 mL), FastDNase I buffer (2 mL), RNA Column (50 ea), 2 mL Tubes (100 ea), Collection Tubes (150 ea), Elution Tubes (50 ea) |
Pack Size | 50 Preps |
Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
Storage | Store at Room Temperature (15-25°C), Dry environment |
Usage Statement | MP Biomedicals的产品仅供研究或进一步生产使用,不得直接用于人体,除非有特殊声明。如需了解更多信息,请联系我们的客户服务部门。 |