Taq CORE Kit 10 Direct Loading - contain MP Biomedicals Taq DNA Polymerase, with a 5'- 3' exonuclease activity, but no 3' - 5' exonuclease activity. Includes: PCR grade dNTPs Mix (10 mM each) |5 x Direct Loading Incubation Buffer with MgCl2 |5 x Direct Loading Incubation Buffer without MgCl2 |Separate 25 mM MgCl2 solution | Both Incubation Buffers contain a densifying agent and a red-purple dye for direct loading onto agarose gel after PCR Cycling. The red purple dye migrates at 400 bp on a 1.2% agarose gel.
Application Notes
The PCR CORE Kits are designed for the amplification of specific regions of DNA using the polymerase chain reaction (PCR). The PCR CORE Kits - Direct Loading - provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Molecular Biology - PCR - Amplification - End-point PCR - Standard PCR
| SKU | 11EPTQKL101 |
| Alternate Names | Taq DNA Polymerase with dNTPs and direct loading buffers |
| Application Notes | The PCR CORE Kits are designed for the amplification of specific regions of DNA using the polymerase chain reaction (PCR). The PCR CORE Kits - Direct Loading - provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel. |
| Base Catalog Number | EPTQKL101 |
| Components | 1x 250 U Taq Polymerase 5 U/uL, PCR Direct Loading buffer with MgCl2 (5x concentrated), PCR Direct Loading buffer without MgCl2 (5x concentrated), MgCl2 at 25 mM and a dNTPs mix at 10 mM each |
| Concentration | Taq DNA Polymerase 5 U/uL and dNTPs at 10mM each |
| Format | Kit |
| Grade | Molecular Biology Grade |
| Pack Size | 250 U |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Protein or Enzyme Type | Molecular Biology Enzymes |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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