Tris Hydrochloride
Application Notes
Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid .
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Biological buffer | Apoptosis
| SKU | 02103130-CF |
| Alternate Names | Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol |
| Application Notes | Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid . |
| Base Catalog Number | 103130 |
| Beilstein Registry Number | 3675235 |
| Biochemical Physiological Actions | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. |
| CAS # | 1185-53-1 |
| EC Number | 214-684-5 |
| Grade | Reagent Grade |
| Molecular Formula | C4H11NO3·HCl |
| Molecular Weight | 157.594 g/mol |
| Personal Protective Equipment | dust mask , Eyeshields, Gloves |
| pKa | 8.08 (25 °C) |
| Purity | 99% |
| Safety Symbol | GHS07 |
| Sterilization of Solutions | Tris solutions can be autoclaved (121°C, 15 psi, 15 minutes) or sterile filtered. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [02103130-CF] lot ID: YC10045](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [02103130-CF] lot ID: YC10045")