SPINeasy™ 微生物DNA提取试剂盒（去除宿主DNA）, SPINeasy™ Host Depletion Microbial DNA Kit，50 preps
宿主DNA会对体液和拭子等含有宿主DNA的样本中微生物组的分子生物学分析产生干扰。SPINeasy host depletion MicrobialDNAkit通过简单便捷的操作流程，从含有大量宿主DNA的样本中去除宿主DNA并快速分离纯化微生物DNA。宿主DNA的去除是通过特殊配方的宿主裂解缓冲液选择性裂解宿主细胞来实现的。通过硅胶柱膜法提取微生物 DNA，可立即应用于下游实验。
提取效果
下图为使用SPINeasy™ Host Depletion Microbial DNA Kit从唾液样本中提取的基因组DNA的PCR结果。结果显示，宿主DNA被有效去除，微生物DNA有效回收。通过qPCR结果分析，估算宿主DNA的去除率和微生物DNA的回收率均高于90%。
图1：使用SPINeasy™ Host Depletion Microbial DNA Kit对唾液样本进行PCR检测。a）使用人类β-球蛋白引物对宿主DNA进行PCR扩增。M：DNA Marker；Lane 1/3/5：未去除宿主DNA直接提取总基因组DNA结果；Lane 2/4/6：使用SPINeasy™ Host Depletion Microbial DNA Kit提取的去除宿主的DNA；Lane 7：PCR阴性对照。
Contact us for your SPINeasy Host Depletion Microbial DNA (SKU:116545050), 5 Preps here. Please provide appropriate information so our local representative are able to get back to you soon.
Application Notes
SPINeasy Host Depletion Microbial DNA Kit通过简单便捷的操作流程，从含有大量宿主DNA的样本中去除宿主DNA并快速分离纯化微生物DNA。
Key Applications
基因组学；分子生物学
| SKU | 116545050 |
| Alternate Names | SPINeasy Host Depletion Microbial DNA Kit; Host DNA Depletion Kit |
| Application Notes | SPINeasy Host Depletion Microbial DNA Kit通过简单便捷的操作流程，从含有大量宿主DNA的样本中去除宿主DNA并快速分离纯化微生物DNA。 |
| Base Catalog Number | 65450 |
| Brand | SPINeasy |
| Components | 12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 µL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes |
| Pack Size | 50 Preps |
| Sample Type | Saliva, Swabs, Bodily Fluid |
| Storage | Store at Room Temperature (15-25℃) in a dry environment |
