Urea,cell culture reagent, 100 g

Name: Urea,cell culture reagent, 100 g
SKU: 02194762.1

SKU: 02194762.1

Synonyms

Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide

CAS Number:

57-13-6

Molecular Formula:

CH₄N₂O

Molecular Weight:

60.06

Beilstein Registry Number:

635724

EC Number:

200-315-5

MDL Number:

MFCD00008022

Packaging size:

100 g

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Specifications

SKU	02194762.1
Alternate Names	Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
Application Notes	Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Base Catalog Number	194762.1
Beilstein Registry Number	635724
Biochemical Physiological Actions	Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Boiling Point	Decomposes at 135 °C (275 °F)(Lit.)
CAS #	57-13-6
Density	1.335 g/mL at 25 °C (Lit.)
EC Number	200-315-5
Grade	Cell Culture Grade
Melting Point	130 - 140 °C
Molecular Formula	CH₄N₂O
Molecular Weight	60.06
Pack Size	100 g
Personal Protective Equipment	Eyeshields, Gloves, Respirator filter
pH	7.5 - 9.5 (7 M aq soln)
Purity	≥98%
RTECS Number	YR6250000
Solubility	Soluble in water (480 mg/mL, 8 M).
Typical Working Concentration	The use of 2 g/L urea in the culture of <i>Kluyveromyces marxianus</i> to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	OD260 nm (7 M aq soln): ≤0.2; OD280 (7M aq soln): ≤0.2
Vapor Pressure	0.000016 hPa at 25 °C (Lit.)

Documents

Datasheet

DS [02194762]