Urea
Application Notes
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins
| SKU | 02194857-CF |
| Alternate Names | Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide |
| Application Notes | Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose. |
| Auto Ignition | Not flammable (USCG, 1999) |
| Base Catalog Number | 194857 |
| Beilstein Registry Number | 635724 |
| Biochemical Physiological Actions | Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. |
| Boiling Point | Decomposes (NTP, 1992) |
| CAS # | 57-13-6 |
| Conductivity | 68.4 uMhos (Lit.) |
| Density | 1.34 at 68° F (USCG, 1999) |
| EC Number | 200-315-5 |
| Format | Powder |
| Grade | Molecular Biology Grade |
| Melting Point | 275° F (NTP, 1992) |
| Molecular Formula | CH4N2O |
| Molecular Weight | 60.056 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| pH | 7.2 (10% solution) |
| Purity | ≥98% |
| RTECS Number | YR6250000 |
| Solubility | >9 [ug/mL] |
| Typical Working Concentration | The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | OD260 nm (7 M aq soln): ≤0.2; OD280 (7M aq soln): ≤0.2 |
| Vapor Pressure | 1.2X10-5 mm Hg @ 25 deg C |
![MP COA [194857] lot ID: 2113A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194857] lot ID: 2113A")