Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Application Notes
Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Counting | Cell Cell Viability | Cryopreservation | Cell dissociation
| SKU | 091689349 |
| Alternate Names | Trypsin 1:250; Trypsin-EDTA solution |
| Application Notes | Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application. |
| Base Catalog Number | 1689349 |
| CAS # | 9002-07-7 (trypsin) |
| Concentration | 10X | 2.5% (w/v) solution |
| Formulation Details | Liquid | Potassium Chloride [KCl]: 400.0000 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 60.0000 mg/L; Sodium Bicarbonate [NaHCO<sub>3</sub>]: 350.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 47.5000 mg/L; Dextrose: 1000.0000 mg/L; Phenol Red Sodium Salt: 17.0000 mg/L; Trypsin 1:250: 2500.0000 mg/L |
| Molecular Weight | Approximately 23;000 or 22000 (for human) |
| Pack Size | 100 mL |
| pH | 5.0 - 6.0 |
| Source | Porcine (for trypsin) |
| Specific Activity | One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified. |
| Specificity | The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. It forms complexes with α2-macroglobulin and can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds. |
| Sterility | Sterile |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1689349] lot ID: 16893009](https://www.mpbiochina.com/static/version1715758459/frontend/Mpbio/default/en_US/images/document.png "MP COA [1689349] lot ID: 16893009")
