Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. This 0.25% Trypsin-EDTA solution is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Dissociation | Cell Counting | Stem Cell Isolation
| SKU | 091689649 |
| Alternate Names | Trypsin (1X), Trypsin-EDTA solution |
| Base Catalog Number | 1689649 |
| CAS # | 9002-07-7 (trypsin) |
| Concentration | 0.25% (w/v) solution | 0.25% Trypsin (1:250) and 1mM EDTA In Modified HBSS Without Ca++ and Mg++ |
| Format | Solution |
| Formulation Details | Liquid | 0.25% Trypsin (1:250) and 1mM EDTA In Modified Hbss Without Ca++ and Mg++ |
| Media Type | Mammalian Media |
| Molecular Weight | Approximately 23;000 (trypsin) |
| Pack Size | 100 mL |
| pH | 7.0 - 7.3 |
| Source | Porcine (for trypsin) |
| Specific Activity | 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units |
| Specificity | Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with α2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds. |
| Sterility | Sterile |
| Unit Definition | One BAEE unit will produce a ΔA253 of 0.001 per minute at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path). One TAME unit hydrolyzes 1 μmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25 °C, pH 8.2, in the presence of 0.001 M calcium ion. One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1689649] lot ID: 16896006](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [1689649] lot ID: 16896006")
