Tris
Application Notes
Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
SKU | 0219455794 |
Alternate Names | Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol;THAM; Tris base |
Application Notes | Tris is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
Base Catalog Number | 19455794 |
Beilstein Registry Number | 741883 |
Boiling Point | 219-220 deg C at 10 mm Hg |
CAS # | 77-86-1 |
EC Number | 201-064-4 |
Format | Crystalline powder |
Grade | Cell Culture Grade |
Hazard Statements | H315-H319-H335 |
Melting Point | 171-172 deg C |
Molecular Formula | C4H11NO3 |
Molecular Weight | 121.136 g/mol |
Pack Size | 5 kg |
Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
pH | pH of 0.1 molar aq soln = 10.4 |
pKa | 8.1 (25 °C)(Lit.) |
Purity | ≥95% |
RTECS Number | TY2900000 |
Safety Symbol | GHS07 |
Solubility | 79.1 mg/mL in <a class="pubchem-internal-link CID-174" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethylene%20glycol">ethylene glycol</a>; 26 mg/mL in <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>; 14.6 mg/mL in anhyd <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 22 mg/mL in 95% <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 14 mg/mL in <a class="pubchem-internal-link CID-6228" href="https://pubchem.ncbi.nlm.nih.gov/compound/dimethyl-formamide">dimethyl formamide</a>; 20 mg/mL in <a class="pubchem-internal-link CID-180" href="https://pubchem.ncbi.nlm.nih.gov/compound/acetone">acetone</a>; 0.5 mg/mL in <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a>; 0.4 mg/mL in olive oil; 0.1 mg/mL in <a class="pubchem-internal-link CID-8078" href="https://pubchem.ncbi.nlm.nih.gov/compound/cyclohexane">cyclohexane</a>; 0.05 mg/mL in <a class="pubchem-internal-link CID-6212" href="https://pubchem.ncbi.nlm.nih.gov/compound/chloroform">chloroform</a>; less than 0.05 mg/mL in <a class="pubchem-internal-link CID-5943" href="https://pubchem.ncbi.nlm.nih.gov/compound/carbon%20tetrachloride">carbon tetrachloride</a> |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
UV Visible Absorbance | OD260 nm (5% aq soln) ≤0.1 |
Vapor Pressure | 2.2X10-5 mm Hg at 25 deg C (est) |