Tris
Application Notes
Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer | Used as a starting material for polymers; oxazolones (with carboxylic acids) and oxazolidines | Used in direct standardization of a strong acid solution
| SKU | 02194855-CF |
| Alternate Names | Trometamol; Tromethamine; Tris-[hydroxymethyl]amino- methane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; THAM; Tris base |
| Application Notes | Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science. |
| Base Catalog Number | 194855 |
| Beilstein Registry Number | 741883 |
| Boiling Point | 219-220 deg C at 10 mm Hg |
| CAS # | 77-86-1 |
| EC Number | 201-064-4 |
| Format | Crystalline powder |
| Grade | Molecular Biology Grade |
| Hazard Statements | H315-H319-H335 |
| Melting Point | 171-172 deg C |
| Molecular Formula | C4H11NO3 |
| Molecular Weight | 121.136 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
| pH | pH of 0.1 molar aq soln = 10.4 |
| pKa | 8.1 at 25 °C(Lit.) |
| Purity | ≥95% |
| RTECS Number | TY2900000 |
| Safety Symbol | GHS07 |
| Solubility | 79.1 mg/mL in <a class="pubchem-internal-link CID-174" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethylene%20glycol">ethylene glycol</a>; 26 mg/mL in <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>; 14.6 mg/mL in anhyd <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 22 mg/mL in 95% <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 14 mg/mL in <a class="pubchem-internal-link CID-6228" href="https://pubchem.ncbi.nlm.nih.gov/compound/dimethyl-formamide">dimethyl formamide</a>; 20 mg/mL in <a class="pubchem-internal-link CID-180" href="https://pubchem.ncbi.nlm.nih.gov/compound/acetone">acetone</a>; 0.5 mg/mL in <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a>; 0.4 mg/mL in olive oil; 0.1 mg/mL in <a class="pubchem-internal-link CID-8078" href="https://pubchem.ncbi.nlm.nih.gov/compound/cyclohexane">cyclohexane</a>; 0.05 mg/mL in <a class="pubchem-internal-link CID-6212" href="https://pubchem.ncbi.nlm.nih.gov/compound/chloroform">chloroform</a>; less than 0.05 mg/mL in <a class="pubchem-internal-link CID-5943" href="https://pubchem.ncbi.nlm.nih.gov/compound/carbon%20tetrachloride">carbon tetrachloride</a> |
| Sterilization of Solutions | Tris solutions can be autoclaved (121 °C, 15 psi, 15 minutes) or sterile filtered. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | OD 260 nm (5% aq soln) ≤0.1 |
| Vapor Pressure | 2.2X10-5 mm Hg at 25 deg C (est) |
![MP COA [194855] lot ID: 1723A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194855] lot ID: 1723A")
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