SPINeasy™ DNA Kit for Yeast，50 preps;SPINeasy™酵母基因组DNA提取试剂盒,50 preps
酵母的细胞壁具有很强的韧性，在实验过程中会面临很多挑战。 在核酸提取过程中，DNA的质量可能会受到多种因素影响，从而影响DNA完整性及纯度。SPINeasy™ DNA Kit for Yeast基于硅胶柱膜法，是专门针对酵母细胞高性能基因组DNA提取的试剂盒。可在短短30分钟内快速从酵母细胞中提取基因组DNA。试剂盒中的结合柱Column S和独特的缓冲液，可提取高产量和高纯度的基因组DNA。提取的DNA可直接用于下游实验，如PCR扩增、限制性内切酶消化和测序等。
Performance
Figure1. 使用SPINeasy™ DNA Kit for Yeast提取的DNA得率与样本量（1×107 - 4×108个酿酒酵母细胞）成正比。上样3 μL洗脱的基因组DNA（100 μL洗脱体积），1%的琼脂糖凝胶电泳分析结果。
Figure 2.
使用SPINeasy™ DNA Kit for Yeast从酿酒酵母中提取的基因组DNA。对其产量和纯度进行评估，与三款不同的竞品试剂盒（Q、T、Z）进行比较。MP的SPINeasy™ DNA Kit for Yeast提取的DNA纯度在最佳的1.8-2.0之间，同时保持了较高的得率。竞品Q和T提取的DNA浓度虽然较高，但A260/230的比值偏低。竞品Z提取的DNA浓度和纯度均偏低。对提取的基因组DNA进行琼脂糖凝胶电泳分析，结果显示，MP提取的DNA条带完整性更高且得率更好。
Application Notes
SPINeasy™ DNA Kit for Yeast基于硅胶柱膜法，是专门针对酵母细胞高性能基因组DNA提取的试剂盒。
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
PCR 扩增、限制性酶消化和测序
| SKU | 116557050 |
| Application Notes | SPINeasy™ DNA Kit for Yeast基于硅胶柱膜法，是专门针对酵母细胞高性能基因组DNA提取的试剂盒。 |
| Base Catalog Number | 6557050 |
| Brand | SPINeasy |
| Components | Lysing Matrix YG (50 x 2.0 mL), Lysis Buffer Y (30 mL), Binding Buffer Y (30 mL), Wash Buffer Y1 (32 mL), Wash Buffer Y2 (4.8 mL), Elution Buffer EB (12 mL), RNase A (280 μL), Column S (50 ea), Collection Tube (50 ea), Elution Tube (50 ea) |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
